Browsing by Author "Muhammad A.Kashmiri"

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    GC-MS and HPLC as the analytical tools in solving the taxonomic controversies of plants
    (2013) Ayesha Mohy-Ud-Din; Muhammad A.Kashmiri; Z. Khan; Muhammad Mujahid; Muhammad H.Q. Khan
    The science of chemical taxonomy is used for the classification of plants on the basis of their chemical constituents which are deeply concerned with the molecular characteristics. Five locally available plant taxa of Solanum nigrum Complex viz.: S. americanum Mill., S. chenopodioides Lam., S. nigrum L., S. retroflexum Dunal and S. villosum Mill. were investigated. GC-MS and HPLC were used as the analytical tools to resolve the international taxonomic controversy about these plants. Comparative qualitative and quantitative analyses of these plant samples were undertaken keeping Alkaloids, Flavonoids and Epicuticular wax as potential characters. The glycosides of alkaloids and flavonoids were determined by HPLC whereas their aglycones and epicuticular waxes were analysed using GC-MS. HPLC and GC-MS analyses of these constituents from S. nigrum Complex had not been reported previously. Statistical cluster analyses of results grouped taxa into different clusters on the basis of similarity index and Euclidean distance.