Browsing by Author "MUHAMMAD AMIN"

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    Development and validation of a method for the estimation of granisetron hcl in pharmaceutical dosage form
    (UMT, Lhr, 2020) MUHAMMAD AMIN
    A rapid and sensitive reverse phase high performance liquid chromatographic method has been established for the estimation and validation of Granisetron HCl in pharmaceutical dosage form. For determination and estimation, C18 column (250 mm × 4.6 mm, 5 µm) was used. The flow rate was set on 1.5 ml/min. The mobile phase was a mixture of potassium phosphate dibasic buffer (pH adjusted to 8.2 by using phosphoric acid), acetonitrile and methanol in the ratio of 45:40:15. The response of detector was linear, after injecting different concentration solutions from 20-120 µg/mL. The value of correlation coefficient was 0.9997. The percentage recovery of Granisetron HCl was calculated 100.33%. This method was observed simple, accurate, and reproducible. The importance of this method is that it can be used commercially as well as in pharmaceutical industries for estimation and quality control analysis of Granisetron HCl in pharmaceutical dosage forms.