Browsing by Author "MAHRUKH FATIMA"

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    STUDY OF ANTIOXIDANT ACTIVITY OF ZIZIPHUS SATIVA
    (UMT.lahore, 2017) MAHRUKH FATIMA
    Ziziphus includes famous specie like ziziphus sativa. Free radicals can cause diseases and also can create damage. It came to know that Ziziphus family is rich in antioxidant amount so its antioxidant activity is amazing. Ziziphus Sativa was extracted by methanol. Then the crude methanolic extract was further separated into four extracts which include ethyl-acetate, chloroform n-hexane and aqueous. The crude extract and all of its fractions were placed screening to study their antioxidant activities. It was done by using DPPH, Phosphomolybdenum and FTC assay methods. In DPPH Assay n-hexane gives 83.61%, chloroform 86.34%, ethyl acetate 91.35% and aqueous 94.90%. Aqueous extract gives the best result according to DPPH assay results. In Phosphomolybdenum assay n-hexane gives 121.81%, chloroform 108.3%, ethyl acetate 113.65% and aqueous 118.45%. According to this method n-hexane gives the best and least results are from chloroform. In FTC assay n-hexane gives 5.23%, chloroform 11.05%, ethyl acetate 10.15% and aqueous 3.73%. Lower absorbance indicates the higher anti-oxidant activity according to this assay. Aqueous is more antioxidant than others and chloroform is the least one here. Results shows that DPPH assay gives the best result as compared to others methods. Hence aqueous and n-hexane portion of the plant extract shows the great radical scavenging activities close to the reference standard BHT.