Browsing by Author "Ghazala Rafique"

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    Phytochemicals from the seeds of peganum harmalal. And their antioxidant and antibacterial activity
    (University of Management and Technology, 2018) Ghazala Rafique
    Peganum harmala L. (P.harmala) is an important medicinal and traditional plant using in variety of diseases belongs to family zygophyllaceae, usually known as harmal, Esphand or wild rue. This research evaluated the phytochemical analysis, in vitro antioxidant activity and antibacterial activity of P. harmala seed extracts against different polarity solvents including methanol, hexane, benzene, chloroform and dichloromethane. Extraction of P. harmala is done by solvent extraction method. A preliminary qualitative phytochemical study showed the presence of secondary metabolites alkaloids, steroids, terpenoids, saponins and glycosides in different extracts of P. harmala seeds while in methanol extract glycosides was absent. Tannins were absent in all extract of P. harmala seeds. Total phenolic content was also determined by Spectrophotometry method by using Folin-Ciocalteu reagent. Antioxidant activity of different extracts of plant seed was checked by using DPPH radical method and ascorbic acid as standard. Dichloromethane extract of plant seed was found to have strong antioxidant activity of 85.4% at 50 µg/mL. Antibacterial activity of plant seeds extracts was also tested by disc diffusion method against three bacterial strains. Chloroform, dichloromethane and benzene extract showed mild antibacterial activity against Salmonella typhimurium while methanol extract showed antibacterial activity against Staphylococcus aureus. Hexane extract failed to inhibit the growth of bacteria. The result have shown that Peganum harmala Linn is rich source of secondary metabolites, a strong antioxidant and possess mild antibacterial activity.