2018

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Browsing 2018 by Author "Ulfat Ali Shah"

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    Isolation and characterization of thermophilic bacteria
    (UMT Lahore, 2018) Ulfat Ali Shah
    In assessing the bacterial diversity present in hot springs, extremotolerent and extremophiles bacteria (need tough environment for growth) were isolated. Several approaches were employed to identify bacteria and not survive in room temperature environment but exist in stressed environment. Samples were collected from Darkot and Hunza valley of Northern area of Pakistan. Temperature up to 4°C to 80°C and pH of neutral medium prior to selecting for extremophile bacteria. Hot springs are the best example of naturally existing extremophelic environment. In our research Bacillus Lichniformis_umt bacterial strain was isolated from the hot spring Hunza Murtazabad and Darkot where bacterial flora is not as much of explored. The thermophilic isolate was screened on the basis of various temperature of 37°C, 50°C and 60°C, and pH of 7 and 9 respectively. The biochemical characterization like sugar fermentation and chemical reactions studied compressively and phylogenetic tree was constructed and Bacillus Lichniformis_umt have 91% homology with neighbor specie strains. The isolate have potential applications in medicine, industry and biotechnology. This grows at optimum temperature of 50°C. It is sensitive to antibiotics of market include Leofloxacin, Erythromycin, HCL monohydrate, Azithromycin and Linofloxacin. This microbe can be a high good source to get industrially important thermostable enzymes