2018

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Browsing 2018 by Author "MASOOMA IQBAL"

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    Extraction purification and characterization of xylanase from bacillus subtilis
    (UMT Lahore, 2018) MASOOMA IQBAL
    Enzymes play spearhead role in many industrial processes and lessen the cost that is why used for many years. Interest in xylanase has increased because of its applications and degrading abilities. It is an enzyme which is commonly found in all organisms such as bacteria, fungi and protozoans etc, whereas the biological system of mammals is unable to produce it. Xylanase hydrolyzes the molecules of the xylan into xylose monomers that are used by many bacteria and fungi as a source of energy. In the present study Bacillus subtilis producing xylanase enzyme were taken for production, purification and characterization. In this study xylanase was isolated from Bacillus subtilis strain and partial purification was done using Ammonium sulphate precipitation. DNS method was used to estimate the activity of the enzyme. Xylan was used as a substrate and xylose standard curve was constructed to check the activity of the xylanase. Cell free culture was saturated with 70% ammonium sulphate, centrifuged at 10,000 rpm and supernatant and precipitates were separated. Cell free sample, ammonium sulphate supernatant, ammonium sulphate precipitates and dialysis samples showed different activities which were 314.6, 245, 5.54 and 2.48 U/mg protein with enzyme activity 5.6, 3.6, 7.5 and 3.96 U/ml. Protein estimation of all the samples showed 178, 140, 21.6 and 14.52 mg/ml of proteins in respective samples. The enzyme was purified to 0.007 folds with 2.14 % recovery. Molecular weight was determined by SDS-PAGE which was 32kDa.