school of Food and Agricultural Science (SFAS)
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Browsing school of Food and Agricultural Science (SFAS) by Author "AZBA SHAFIQ"
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Item EVALUATING THE EFFECTIVENESS OF SAPONINS AGAINST BIOFILMS FORMATION(UMT.Lahore, 2021-07-14) AZBA SHAFIQFood safety enroots deeply in food industry. The presence of multi-drug resistant bacterial strains lead to the formation of biofilm within the food chain that causes serious threat to food safety and consumer health. The study aimed at the replacement of traditional antibacterial strategies by application of saponins that are secondary plant metabolites as potential bacteriocidal agents and bacterial biofilms destructors. Biofilm being the highly resistant microbial matrix is of particular concern. S.aureus being the multi-drug resistant and potential biofilm former is targeted in the current study. The saponins were extracted from four different plant samples licorice, quillaja, quinoa and alfalfa using the different ethanol concentration from 0-100 % (w/w). The concentration of saponin and protein content was estimated using spectrophotometric studies. The different plants required different optimal ethanol concentrations for maximum saponin yield and extraction. The optimal conditions, composition and genetic basis of bacterial biofilms was also evaluated in this study. The bacteriocidal action was evaluated using three different methods of well-diffusion, direct pouring and filter disc method. However, the antibiofilm activity was estimated by the application of 100 μL and 200 μL of saponins extracted using different ethanol percentages. The results indicated that saponins tend to have detergent like attributes that improves the permeability of bacterial cell-membranes thus effecting the role of antibacterial treatment however saponins have no antibacterial effect while the antibiofilm activity was observed for licorice and alfalfa saponins.