2023

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Browsing 2023 by Author "SADIA ASHRAF"

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    Synthesis of Cellulose-Based Molecularly Imprinted Polymers for Creatinine Sensing
    (UMT, Lhr, 2023) SADIA ASHRAF
    Ensuring precise and dependable analysis of creatinine is crucial for promptly identifying and efficiently monitoring kidney disease in patients. To address this pressing demand, the present study focuses on developing a molecularly imprinted polymer (MIP) tailored for the detection of creatinine, designated as MIPCre. The methodology employed revolves around a cost-effective and highly selective technique utilizing metal-free Organic Photoredox-Catalyzed Atom Transfer Radical Polymerization (O-ATRP). This novel process employs a hydroxypropyl cellulose (HPC)-based macroinitiator in conjunction with methacrylic acid (MAA) as the functional monomer. The creation of MIPCre is achieved through O-ATRP, a procedure catalyzed by 4CzIPN under visible light irradiation. The significance of this approach lies in its ability to offer an efficient, easily preparable, and economically viable solution for generating molecularly imprinted polymers tailored for creatinine detection. MIPs possess inherent advantages such as reusability and high selectivity for the target molecule, attributes that are particularly desirable in clinical settings where accuracy and reliability are paramount. The synthesis procedure entails the production of a range of poly(methacrylic acid) grafted HPC (PMAA-g-HPC), which undergoes thorough characterization employing diverse analytical methods such as scanning electron microscopy (the SEM), Fourier-transform infrared (the FTIR) spectroscopy, and thermogravimetric analysis (the TGA). Significantly, the utilization of this pioneering surface-MIP approach leads to notable progressions, including achieving an exceptionally low limit-of-detection (LoD) of 6.2 µg/mL, accompanied by a broad detection range spanning from 0.1 to 14 µg/mL. These findings underscore the efficacy and versatility of the proposed methodology in sensitively detecting creatinine within a clinically relevant concentration range. Moreover, the practical applicability of the newly devised MIP sensor is assessed through extensive creatinine measurements, demonstrating its potential efficacy as a rapid, accurate, and point-of-care diagnostic instrument for monitoring kidney disease in patients Expanding beyond the technical aspects, the implications of this research extend to broader implications within the realm of healthcare and diagnostics. The development of a reliable, cost-effective, and selective creatinine sensor holds profound implications for enhancing patient care, particularly in contexts where access to sophisticated laboratory infrastructure may be limited. By enabling the early detection and continual monitoring of kidney function, the MIP sensor possesses the potential to facilitate prompt intervention and customized treatment approaches, ultimately enhancing patient outcomes and alleviating the burden of kidney disease on healthcare system. Moreover, the versatility of the MIP sensor platform extends beyond creatinine detection, with potential applications in diverse fields such as environmental monitoring, food safety, and pharmaceutical analysis. The adaptability of the synthesis methodology opens avenues for future research and innovation, including the exploration of novel MIP formulations targeting different analytes and the integration of advanced sensor technologies for enhanced performance and usability. In conclusion, this study signifies a notable advancement in the field of molecularly imprinted polymer-based sensors for detecting creatinine, presenting a hopeful approach to meet the urgent requirement for precise and readily available diagnostic instruments in managing kidney diseases. Through interdisciplinary collaboration and continued technological advancement, the MIP sensor holds the potential to revolutionize clinical diagnostics and improve patient care on a global scale.