2020

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Browsing 2020 by Author "Mehwish Andleeb"

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    Immunoinformatic-based screening of immunogenic fragments from the pathogenic proteins of NAEGLERIA FOWLERI against the HLA-alleles of majorly affected population
    (UMT Lahore, 2020) Mehwish Andleeb
    Naegleria fowleri is a unicellular pathogenic amoeboid flagellate. The trophozoite phase is responsible for causing primary amoebic meningoencephalitis, with the 98% fatality rate. Considering the complex pathogenicity, as well as the resistance towards most of the antifungal drugs, present study aimed to propose a multi-epitope ensemble against N. fowleri restricted to targeted populations. The significant B-cell and T-cell epitopes from all five immunogenic surface proteins, such as Mp2CL5 (181), HSP70 (659), Naegleria Pore A (307), Naegleria Pore B (484), and NF23 (229), were selected. The immunogenicity and allergenicity of selected epitopes were analyzed using VaxiJen and AllerTop v. 2.0. The binding affinities of selected T-cell epitopes with the South Asian, USA, and Australian specific HLA alleles, retrieved through AFND, were evaluated through the IEDB server. The antigenic fragments with the highest binding affinity towards these alleles were analyzed. The nominated epitopes were covering 94.53% for the USA, 84.27% for Australia, and 73.77% for the South Asian region. The structural modeling through I-TASSER and validation via SAVES server v.5.0 suggested all these proteins were stable. The common T-cell epitopes from all these populations were labeled using the PyMol graphic tool. The immunogenicity, non-allergenicity, and maximum population coverage of short-listed epitopes marked them as efficient candidates for vaccine designing.