2023

Permanent URI for this collection

Now showing 1 - 1 of 1

In-silico tripartite interaction analysis of bacterium, insect and plant in citrus greening or huanglongbing disease
(UMT, Lhr, 2023) Muhammad Awais
Citrus greening disease which is also known as Huanglongbing (HLB), it caused serious threat to the citrus industry across the globe due to its rapid spread. it is caused when tripartite association occur between psyllid, bacteria and hots plant. it infects the citrus plants through vector Asian citrus psyllid (ACP) also known as Diaphorina citri Kuwayama (D. citri) which carry the infectious bacterium Candidatus Liberibacter asiaticus (CLas), which is gramnegative bacterium. During infection the fruits become smaller, taste sour, and bitter and sometime cause the death of plant. The aim of this study was to access the potential treatment of HLB, for this approach we conducted the in-silico experiment. Where we docked the maximum number of proteins of D.citri and CLas with citus plant’s phytochemicals, Density functional theory (DFT) of phytochemicals was performed to check the reactivity of those phytochemicals which showed best binging energies with maximum number of proteins of psyllid and CLas. Three phytochemicals, Eriocitrin, Rhoifolin and Narirutin showed beast binging affinities as compare to the rest