2024

Permanent URI for this collection

https://escholar.umt.edu.pk/handle/123456789/10862

Browse

Browsing 2024 by Author "HUZAIFA AHMED"

Now showing 1 - 1 of 1
  • Thumbnail Image
    Item
    Antibiogram profiling of pathogenic bacteria isolated from food and water sources in lahore
    (UMT Lahore, 2024-08-26) SYEDA IQRA SEHAR; HUZAIFA AHMED; JAVERIA KHAN
    Food and water safety and consumption are facing a significant problem due to foodborne bacteria that are resistant to drugs. The goal of this study was to identify prevalent food and waterborne pathogens from locally available food and water sources in Lahore, along with a profile of resistance and susceptibility to antimicrobial drugs. This study aimed to investigate, isolate, and identify food and waterborne pathogens and analyzing their antibiogram profile, achieving SDG targets related to clean food and water and also sanitation( SDG 3 and 6). We collected 53 food and water samples (30 and 23, respectively), processed them through serial dilution and segregated 55 isolates. The isolates were identified by Gram staining and Biochemical tests. Common food and waterborne pathogens discovered were Klebsiella, Serratia, Salmonella, Citrobacter, Providencia, and Escherichia. Areas with majority of contaminated food and water were PIA Road Food Market (14 pathogenic isolates), UMT Road Food Market (7 pathogenic isolates), and College Road (4 pathogenic isolates). Antibiogram analysis was performed by introducing these isolates to certain antibiotics i.e. Azithromycin, Ampicillin, Chloramphenicol, Cefotaxime, and Ciprofloxacin. This analysis showed that Bacteria such as E. coli, Serratia, Proteus and Salmonella were 100% resistant to all antibiotics whereas, Providencia, Klebsiella and Citrobacter were 98, 96 and 91% resistant to chloramphenicol and azithromycin. The most prominent bacteria in our samples was Citrobacter whose susceptibility is as follows: Amoxicillin (0.6+1.1), Ciprofloxacin (15+2), Azithromycin (10+2), Chloramphenicol (15+2) and Cefotaxime (3+2). These findings underscore the necessity for improved water and food safety measures and public education on hygiene practices. As Pakistan grapples with the dual challenges of infectious diseases and rising antibiotic resistance, this research provides actionable insights that can drive policy reforms and bolster health care responses.