Quantification of touch DNA from mobile surfaces using three manual DNA extraction methods
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Date
2020
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UMT Lahore
Abstract
Applications of forensic science resolve legal matters that are in physical, natural, and social discipline. Offenders are getting up to date with innovative technology, where the use of the mobile phone is very common, nowadays. A big leap is to overcome cold cases with inexpensive, finer, and less time taking ways. The purpose of this study was Quantification of touch DNA from mobile surfaces using three manual DNA extraction methods towards. The genuine idea of touch DNA and contact DNA proof has stayed difficult to track down, where three protocols further process the shed cells to get better yield; Phenol Chloroform, Alkaline Lysis, and Trehalose. In this study, DNA extracted by the results of these manual organic methods and spectrophotometer used for quantification of male and female DNA samples from different mobile surfaces; Glass, Plastic, Metal, and Rubber. Where the plastic and glass surfaces were showing more fine extracted quantity than metal and rubber. This research was to evaluate the best DNA extraction method for contact DNA, having better quantification. Overall, Alkaline lysis showed distinguishable results for DNA concentration than Trehalose and PCI. Whereas PCI is more, appropriate for purification. Hence, it was the Alkaline lysis method observed with a higher concentration of DNA mark for a DNA profile.