Antimicrobial activity of nigella sativa plant leaves
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Date
2018
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UMT Lahore
Abstract
In recent century, chemicals that used as anti-agents in medicines have become less active and more toxic. Due to this reason herbs have become vital source of clinical medicines because they have more resistant against organisms and are less toxic. Nigella sativa is a medicinal plant belong to family Ranunuclease. It contain many certain phytochemicals that exhibit activities i.e. antibacterial, antidiabetic, antifungal etc. This plant have been widely used to cure diseases. In this research, antimicrobial activity of N. sativa plant leaves Ethanolic extract was identified against bacteria (Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa & Aspergillus niger). Three antimicrobial techniques were applied: agar disk diffusion method, Agar well diffusion and Microdilution assay. Extract showed inhibition against gram negative bacteria but no inhibition seen in case of B. subtilis & A. niger. The extract did not inhibit the growth of gram positive bacteria. E. coli and P. aeruginosa has shown growth on media in presence of the extract. This study narrated that phytochemicals in leaves of Nigella sativa have potential to resist growth of gram negative bacteria. After further in vitro and in vivo trials, such extract can be used to treat ailments in animals and humans.