Development and validation of analytical method of silymarin by RP-HPLC with UV detector

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dc.contributor.authorAslam, Sohail
dc.date.accessioned2016-04-13T10:08:28Z
dc.date.available2016-04-13T10:08:28Z
dc.date.issued2015
dc.descriptionSUPERVISOR: DR. Sammia Shahiden_US
dc.description.abstractMilk Thistle (Silybum marianum) is a wild, herbaceous plant. Generally the extract of Silybum marianum is expressed as total Silymarin. Silymarin is composed of flavonolignans, silybin, silydianin and silychristin. Out of these compounds, silybin is considered the most vital as it plays an important role for the treatment of liver disorder. Silymarin is very important natural drug which is widely used for the treatment of variety of diseases, especially for hepatic disorders. A novel, reliable and a sensitive method to determine the amount of Silymarin was developed by using reversed phased HPLC method with isocratic solvent delivery. Silymarin was separated using Hypersil ODS C18 column maintaining temperature at 40°C in the column oven and detector was set at wavelength 288 nm with mobile phase in a ratio Methanol:Water:Phosphoric acid (46:54:0.1 v/v/v). The silymarin was tested with respect to silybin which is the most vital and pharmacological active substance. For this purpose the retention time of the silybin among all flavonolignans were determined by injecting silybin standard. Then the silymarin standard and silymarin raw material sample were injected and successfully determined the assay of silymarin. The developed method was then validated as per ICH guidelines. Linearity, accuracy, precision and robustness are the key parameters used to validate the method. This method could easily be used for the determination of silymarin for routine analysis of raw material to maintain its quality and to check the efficacy of the incoming consignments of raw material.en_US
dc.identifier.urihttps://escholar.umt.edu.pk/handle/123456789/1767
dc.publisherUNIVERSITY OF MANAGEMENT AND TECHNOLOGYen_US
dc.subjectMS Thesisen_US
dc.subjectValidation of silymarinen_US
dc.subjectSilybum marianumen_US
dc.subjectRP-HPLC WITH UV detectoren_US
dc.titleDevelopment and validation of analytical method of silymarin by RP-HPLC with UV detectoren_US
dc.typeThesisen_US
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