MAIRA NAEEM2025-11-172025-11-172020https://escholar.umt.edu.pk/handle/123456789/10321Thymosin alpha 1 is a hormone consists of 28 amino acid and play role in treatment of diseases because of its immune stimulation, anti-viral and anti-cancer properties. p28 is a peptide derived from bacterial azurin protein and have anti-tumor activity. T-1 and p28 are both fused with the help of GGGGS linker. The objectives are to clone the fusion protein in E. coli DH5 cells. TA cloning is specifically used for the process cloning and other steps of cloning have been followed by using kit method and plasmid pTZ57R/T was used. The fusion protein expects to be a strong candidate for cancer therapy because it is not only beneficial in stimulating immune response but it also targets tumor cells and block its way to escape from immune surveillance.enThesis