MUHAMMAD AZEEM BHATTI2025-12-012025-12-012016https://escholar.umt.edu.pk/handle/123456789/14328Background: Pseudomonas is a gram negative aerobic bacterium of relatively low virulence and belonging to the bacterial family pseudomonadaceae. Pseudomonas is abundant in moist environment, mostly as waterborne and soil borne organisms. Pseudomonas species have been found in water, plants, soil and animals. Pseudomonas aeruginosa colonization apparently occurs in more than 50% of humans. Pseudomonas is an opportunistic pathogen, causing nosocomial infections. These organisms show innate resistance to many antibiotics and can develop new resistance after exposure to antimicrobial agents. It causes respiratory system infections, blood infections, wounds infections, urinary tract infections and variety of systemic infection in patients. Methods: The blood, wound and urine sample collected from different patients. The blood sample was collected blood culture bottle. In the laboratory all sample were culture aerobically through blood agar and MacConkey agar plates for 24h at 37 0c temperature. The prevalence and resistance of antibiotic determine through catalase test, oxidase test. Results: Total 800 samples were collected in clinical sample and 234 samples give positive test against pseudomonas specie. Four different infection site samples were collected in clinical patients, wound, blood, upper respiratory tract, urinary tract infection. Pseudomonas was found 39.74% in wound infection, 46.86% in upper respiratory tract infection, 9.4% in blood infection and 4.7% urine infection. Twenty different types of antibiotics use against this type of infection which cause Pseudomonas specie. Pseudomonas specie was highly prevalence in upper respiratory infection and wound infection as compare with urine and blood infection. The antibiotic resistance rate varies in different types of infection with same antibiotic. Mostly resistance lies in upper respiratory tract and wound infection. The Ceftriaxone showed 92% resistance, Ciprofloxacin showed 68%, Levofloxacin showed 59%, Cefixime showed 98%, Amoxicillin showed 100% resistance. Minocycline showed 91% Tigecycline showed 94%, Meropenam showed 81%, susceptibility against pseudomonas species. The results showed that the Pseudomonas specie give maximum resistance against Ceftriaxone, Ciprofloxacin, Levofloxacin, Cefixime, Amoxicillin and Piperacillin Conclusion: Total 800 samples were collected from different clinical sources and 234(29.24%) samples give positive test against Pseudomonas specie. Pseudomonas specie showed maximum infection in upper respiratory tract (46.86%) and wound infection (39.74%). Amoxicillin antibiotic showed (100%) resistance against pseudomonas specie. It can be concluded in study that single antibiotic showed different results at different infection sites.enThesis