MUQADAS2025-11-172025-11-172020https://escholar.umt.edu.pk/handle/123456789/10313Breast cancer has become the major issue and the most common type of invasive cancer worldwide among the women. Plant based treatment of breast cancer is considered safe because plants do not affect surrounding part of body. Rhazya stricta is a plant that is seem to have many alkaloids, flavonoids and many other phytochemical contributing for its potential to cure many diseases. Aim of this study is to evaluate the anticancer potential of R.stricta and its effect on de novo lipid biosynthesis pathway in MCF-7 breast cancer cell line. In current study, methanolic extract of R.stricta was used. A range of plant extract dilutions (0µg/ml-300µg/ml) applied on MCF-7 cells. MTT assay performed to check the cell viability and to determine IC50 value of extract. Then MCF-7 was treated with IC50 value of dose i.e. 25µg/ml for 72 hours’ incubation. Furthermore, expression analysis of FASN and ELOVL6 genes evaluated in the treated cells that were seem to down-regulate after treatment with methanolic extract of plant.enThesis