Muhammad Usama Yaqoob2025-11-172025-11-172020https://escholar.umt.edu.pk/handle/123456789/10348Lr34 is a leaf rust resistance gene of hexaploid wheat Triticum aestivum L. This wheat is also known as the bread wheat as it is the source of food for humans and also feeding stuff for the animals. 95% of the world wheat cultivated constitutes hexaploid wheat which is Triticum aestivum L. It is the main host of leaf rust pathogen called Puccinia triticina. Lr34 gene provides resistance against the urediniospore's life stage of Pt. Some studies show that the locus of LR34 is on 7D chromosomes while other homologs are present on other chromosomes. This study involves various in silico techniques to carry out the analysis, characterization, structural and functional predictions, and the phylogenetic and evolutionary relationship of the LR34 protein encoded by the Lr34 gene. Various computational tools were used for the predictions and verification of the results. NCBI was used for the data and the sequence retrieval which showed that the protein contains 1402 amino acids and has an accession ID ADK62371. Other tools that were used in this study include Scanprosite, InterPro, NCBI CDD, SOPMA, DNAssist, I-TASSER, SWISS MODEL, SAVES 5.0, and MEGAX. PyMOL and Chimera were used for visualization. From different domain search tools, it was found that LR34 contains the domains of the ABC transporter family. Out of three NCBI, CDD predicted the conserved domain region from amino acod#43 onward. While the sequence homology and the phylogenetic analysis confirmed hypothetical protein CFC21_054641 [Triticum aestivum] as the nearest neighbor and homolog of LR34 in the evolutionary history. I- TASSER predicted the model which had only 6% residues in the disallowed region in the Ramachandran plot while the rest were in the most favored, additional allowed, and generously allowed regions. To make the model more favorable the model was further energy minimized. It was found that the protein is involved in oxidoreductase reaction more specifically in fatty acid catalysis and is an obsolete intracellular part.enThesis