Sana Ashraf2025-12-082025-12-082023https://escholar.umt.edu.pk/handle/123456789/15547Mushrooms are known to mankind since early human civilization and gradually gained importance due to their medicinal and nutritional properties. These are also origin of mineral nutrients, proteins and carbohydrates Mushrooms are important fungi used in complementary medicine. Helvella spp. are found most abundantly in Himalayan moist temperate forests of Pakistan. Native people are using these mushrooms since centuries. For the first time, mineral analysis and anti-oxidant activities of Helvella spp. have been reported from Pakistan. This study gives attention to expose their mineral profiles by Flame photometery, Atomic absorption spectroscopy and Spectrophotometry. Biological activities focusing on antioxidant activities based upon lyophilized sequential extracts and traditional preparations attain from the fruit bodies of these mushrooms. The present study revealed that Helvella acetabulum are most enrich in sodium (53mg/100mg) and Helvella paraphysiorquata showed highest trace element iron (0.51mg/100mg) concentration. The most active oxygen inhibitor and enzyme inhibitory extracts were shown by H. paraphysitorquata. Information obtained from this data reveals that H. paraphysitorquata used as raw materials in food, pharmaceutical industries, nutraceutical and biotherapeutic. The results suggested that mushrooms can be ingested as a functional food and employed as a therapeutic element due to its bioactive substances and its well-balanced nutritional profile. Hopefully the selected mushroom species will further be analyzed and used it in daily diet to reduce the deficiency of micronutrients in people.enThesis