Maryam Saleem2025-11-182025-11-182019https://escholar.umt.edu.pk/handle/123456789/10520Diabetes mellitus (DM) is a group of metabolic diseases designated by hyperglycemia resulting from defects in insulin action, insulin secretion, or both. This disease is a worldwide medical health issue and has an enormous effect on health systems. The epidemic of DM and its complications represents a noteworthy health risk globally. Clearly, hyperglycemia performs a vital role in diabetes advancement and succession alongside other microvascular diabetic problems. In this study, we evaluated the expression of apoptosis-associated genes, BCL-2 and BAX in the glucoseinduced NIH cells and we investigated the association of NIH cells with hyperglycemia and relationship between hyperglycemia and apoptosis. The role of glucose on viability of cells was also investigated and the toxic effect of glucose on NIH cells was checked. We selected fibroblast NIH/3T3 cell line for experiments; half of them were induced by 30mM glucose while the others served as controls. The expressions of Bcl-2 and BAX were measured using PCR and MTT assay was utilized to determine amount of viable cells. The results showed that the expression of Bcl-2 was decreased in glucose-induced NIH cells than that of the control, whereas the expression of BAX in glucose-induced NIH cells was increased than that of the control. The results also revealed that all glucose-induced cells have reduced cell viability in time dependent manner. Prolonged hyperglycemia induces apoptosis in the NIH cells; in which apoptosis plays a vital role possibly by an increase in the BAX expression and decrease in Bcl-2 expression. As glucose-induced apoptosis increases thus supporting that glucose is toxic for NIH cells.enThesis