SABAHAT MAHNOOR2025-11-182025-11-182020https://escholar.umt.edu.pk/handle/123456789/10492Composting is an environment friendly and most economical method to decompose the organic waste by biologically active degraders which are naturally present in the municipal solid waste. Due to increase in world population the rate of pollution has also been increased. Compost also produce essential components (K, P, N) which help in amendment of soil. Composting carried out under the ideal conditions of temperature, pH, nutrients level and lignin content. Soil plays a crucial role to produce microorganism (Bacteria, Fungi) which help in decomposition of waste. This study is designed to isolate and identify the efficient bacteria at mesophilic stage of composting which works best on optimum temperature of 35°C. Bacterial strain “X” isolated from mesophilic stage of composting and optimised. Activity of bacterial strain “X” was observed on blood agar and three different carbohydrate media. Optimization was carried out by using spectrophotometry and electrical impedance techniques. Literature revealed that bacterial species present in compost pile always show best degradation capabilities. This study is designed to isolate the most efficient bacillus strain. This strain has best potential and it is able to degrade more efficiently in a very short duration of time.enThesis