Browsing by Author "MUHAMMAD MOHSIN SIDDIQUE"

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    Method development and validation for estimation of dexlansoprazole delay release capsule in pharmaceutical dosage form
    (UMT, Lhr, 2021) MUHAMMAD MOHSIN SIDDIQUE
    A simple and precise method has been developed and validated for the assessment of Dexlansoprazole in pharmaceutical dosage form as per International Council on Harmonisation (ICH) guidelines by reverse phase high pressure liquid chromatography. For estimation ๐ถ18 (4.6 mm x 250 mm x 5 ยตm) column was used and wavelength of UV/VIS detector was set on 285 nm. Flow rate of 1 mL/min was set in isocratic mode with run time adjusted to 10 minutes. The mobile phase was a mixture of ๐พ2๐ป๐‘ƒ๐‘‚4, acetonitrile, and methanol in the ratio of 45:40:15 v/v. For estimation of Dexlansoprazole some system suitability parameters have been checked like tailing factor of peak that was experimentally obtained as 1.19 % and relative standard deviation of 0.3 % that was calculated from the area under the curve of the peak, in addition to this 5780 number of theoretical plates was obtained. Dexlansoprazole showed linear flow rate and retention time of 5.56 minutes in the range of 20-200 ppm .The correlation coefficient of ( ๐‘…2) 0.999 was obtained from the linear curve. Around 99.8 % of Dexlansoprazole was recovered experimentally from commercially obtained sample of Dexiva Capsule 60 mg. The limit of detection (LOD) was obtained as 13.67 ยตg.mL-1 and limit of quantification (LOQ) of about 41.42 ยตg.mL-1. Similarly some other parameters like precision, linearity, system suitability, accuracy, recovery, correlation coefficient, ruggedness also used for the method development and validation for the estimation of Dexlansoprazole. This development and validation method can be used commercially as well as in pharmaceutical industries for the estimation of Dexlansoprazole in pharmaceutical dosage form. This method is very precise and sensitive as compared to previously reported methods for estimation of Dexlansoprazole.
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    SYNHTESIS AND SPECTRAL CHARACTERIZATION OF CARBOXYLATE LIGAND DERIVED FROM p-TOLUIDINE
    (UMT.lahore, 2017) MUHAMMAD MOHSIN SIDDIQUE
    Many carboxylate ligands are derived from various amines and aromatic compounds. The carboxylate ligand that I have prepared is by reacting maleic anhydride with p-Toluidine which produces a light brown initial color and light yellow end product after continuous stirring on magnet stirrer overnight. It has very high melting point and is soluble and insoluble in different solvents. Its physical and chemical properties are tested by using different solvents like ethyl acetate, acetone, methanol, ethanol, chloroform, n-hexane, DMSO, distilled water, glacial acetic acid. After obtaining the product I have calculated the %age yield of the product that I have obtained and characterized this carboxylate ligand by using FT-IR. This ligand have many applications as an intermediate, as antifungal, anti-bacterial active compound, antimicrobial compound, in the synthesis of MOF`s, as anti-drug, also as active catalysts.